Composite

Part:BBa_K4398010:Design

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-10-11)


Hygromycin resistance transcriptional unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2653
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2653
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
    Illegal BglII site found at 487
    Illegal XhoI site found at 960
    Illegal XhoI site found at 1044
    Illegal XhoI site found at 1316
    Illegal XhoI site found at 1606
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2653
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2653
    Illegal NgoMIV site found at 2406
    Illegal AgeI site found at 1124
    Illegal AgeI site found at 1844
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ATG start codon comes from the fusion sites between the promoter and coding sequence


Source

Parts BBa_K4398000; BBa_K4398001; BBa_K4398005

References